A new polymorphic probe on chromosome 3p: λLIB24–12′ (D3S727)

A new polymorphic probe on chromosome 22: NB84 (D22S183).

Source/Description: NB84 is a 1.0 kb Hindm-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and is subcloned into pUC9. Polymorphism: PstI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: 4.6 kb (Al) and 4.0 kb (A2). No constant bands were present. Chromosomal Localization: Regional localization...

A new polymorphic probe on chromosome 22: NB17 (D22S181).

Source/Description: NB17 is a 0.7 kb HindlO-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and was subcloned into pUC9. Polymorphism: Bgin or TaqI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: Bgin (Al: 12.5 kb, A2: 4.0 kb) with a constant band at 3.0 kb; TaqI (Bl: 2.9 kb, B2: 2.2 kb). The ...

Cloning of the esterase D gene: a polymorphic gene probe closely linked to the retinoblastoma locus on chromosome 13.

The study of recessive oncogenes such as those responsible for retinoblastoma and Wilms tumor is difficult because the gene products involved are unknown and because the diseases are not associated with unique cellular or molecular phenotypes suitable for genetic manipulation. Since the gene for esterase D (ESD) is known to be tightly linked to the retinoblastoma locus (RB1) in the q14.1 band o...

YM40 (D2S75) is a polymorphic DNA sequence on chromosome 2.

Transgene with Polymorphic A Bacterial Artificial Chromosome